霉菌酸性蛋白酶高产突变菌株9169的选育

对一株宇佐美曲霉进行紫外光、微波和60Co的逐级诱变育种,使酸性蛋白酶产量从2800U/mL提高到7200U/mL.突变株传代多次,产酶性能保持稳定;对突变株与出发株的发酵过程进行了比较研究,发现突变株最大产酶时间提前10h以上,而培养基pH值对产酶有一定影响.     

中国人共刺激信号抑制配体表达载体的构建及在大肠杆菌DH5α中的表达

构建CTLA－4基因表达载体并在大肠杆菌DH5α中表达CTLA－4蛋白。方法：用PCR方法获得中国人CTLA－4基因第二外显子，构建重组表达质粒pPBCTLA，转化大肠杆菌DH5α，以PCR和RFLP方法筛选阳性克隆，以免疫印迹技术检测表达产物。结果：阳性重组子在DH5α中经温度诱导表达CTLA－4蛋白，分子量与理论值相符（约12kD)，进一步分析表明，CTLA－4蛋白主要以包涵体形式存在。Western-blot结果证实，12k的表达条带可与标准羊抗人CTLA－4ITgG起特异反应。淋巴细胞转化实验结果表明该蛋白具有明显的免疫抑制作用。结论：用基因工程技术获得中国人CTLA－4基因表达蛋白，为进一步研究CTLA－4的生物学功能及研制CTLA－4抗体奠定了基础。     

舌癌与瘤内血管形成相关性研究

目的　研究瘤内血管密度 (MD)与舌癌淋巴结转移、复发以及对预后的影响。方法　采用免疫组化SP法FⅧ因子多克隆抗体对 36例舌癌、瘤内血管进行标记分析。结果　颈淋巴结转移组 (19例 )MD31 35± 4 42 ,明显高于无转移组 (17例 )MD15 .12± 5 .34(P <0 .0 1)。随访发现 ,MD高表达组与低表达组颈清术后复发率分别为 6 0 %和12 5 % (P <0 .0 1)。舌原发灶复发 8例MD为 45 .5 7± 6 .2 6 ,比无复发组 2 8例MD2 0 .35± 4.13高二倍多 (P <0 .0 1)。结论　肿瘤内血管形成与舌癌的复发转移密切相关 ,而与病理分级及临床分期无关。因此瘤内MD可作为判定舌癌预后的独立指标。     

AMPK‐dependent signaling modulates the suppression of invasion and migration by fenofibrate in CAL 27 oral cancer cells through NF‐κB pathway

Fenofibrate, a peroxisome proliferator‐activated receptor alpha (PPARα) agonist and lipid‐lowering agent, has been used worldwide for treatment of hyperlipidemia. The clinical trials demonstrate that fenofibrate possesses multiple pharmacological activities, including antitumor effects. However, the precise mechanisms in oral squamous cell carcinoma (OSCC) remain unclear. In this study, we investigated the anticancer effects of fenofibrate on the migration and invasion of human oral cancer CAL 27 cells. Fenofibrate inhibited the cell migration and invasion of CAL 27 cells by the wound healing and Boyden chamber transwell assays, respectively. In addition, fenofibrate reduced the protein expressions of MMP‐1, MMP‐2, MMP‐7, and MMP‐9 by Western blotting and inhibited enzyme activities of MMP‐2/‐9 using gelatin zymography assay. Results from immunoblotting analysis showed that the proteins of p‐LKB1 (Ser428), LKB1, p‐AMPKα (Thr172), p‐AMPKα1/α2 (Ser425/Ser491), p‐AMPKβ1 (Ser108), and AMPKγ1 were upregulated by fenofibrate; the levels of p‐IKKα/β (Ser176) and p‐IκBα were reduced in fenofibrate‐treated cells. Also, fenofibrate suppressed the expressions of nuclear NF‐κB p65 and p50 by immunoblotting and NF‐κB DNA binding activity by EMSA assay. The anti‐invasive effect of fenofibrate was attenuated by compound C [an adenosine 5′‐monophosphate‐activated protein kinase (AMPK) inhibitor] or dominant negative form of AMPK (DN‐AMPKα1). Thus, fenofibrate considerably inhibited metastatic behaviors of CAL 27 cells might be mediated through blocking NF‐κB signaling, resulting in the inhibition of MMPs; these effects were AMPK‐dependent rather than PPARα signaling. Our findings provide a molecular rationale, whereby fenofibrate exerts anticancer effects and additional beneficial effects for the treatment of cancer patients. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 866–876, 2016.     

Design and application research of nucleotide sequence analysis system software

INTRODUCTION　　From integrallevel and celllevel,the research of organism and diseasediagnosishave already entered molecular level.Molecular biology is the science to researchstructure and function of organism macromolecule.Based on differentgene code se-…     

奇静脉逆行灌注在主动脉手术中对脊髓保护的解剖学研究

目的 :为主动脉手术中脊髓保护提供形态基础和术式设计。方法 :解剖观察了 3 1例成人标本的奇静脉系、椎静脉丛 ,以及脊髓静脉回流途径。结果 :观测奇静脉系及相关静脉 ,其直径为 ( x±s) :奇静脉为 (9.2± 1.9)mm ,半奇静脉为 (5 .5± 1.2 )mm ,副半奇静脉为 (3 .8± 0 .9)mm ,左最上肋间静脉为 (2 .0±0 .4)mm ,右最上肋间静脉为 (1.9± 0 .4)mm ,左腰升静脉为 (2 .3± 0 .5 )mm ,右腰升静脉为 (2 .2± 0 .6)mm。根据奇静脉属支的配布和肋间静脉汇入形式 ,将奇静脉系分为 4种类型。奇静脉及其属支中存在静脉瓣 ,有个体差异。脊髓静脉密集 ,与椎内静脉丛有丰富的网络连接 ,并通过神经根静脉与椎外静脉丛、奇静脉系相交通。结论 :在主动脉手术中应用奇静脉逆行灌注脊髓保护 ,是一种行之有效的新术式。     

微创手术加尿激酶血肿清除治疗高血压性脑出血对照研究

目的探讨微创手术加尿激酶血肿清除在高血压性脑出血治疗中,微创手术加尿激酶血肿清除治疗组,对改善脑出血预后的机制。方法34例符合手术适应征的高血压性脑出血急性期患者被随机分为微创手术加尿激酶血肿清除治疗组和常规手术对照组,比较两组治疗后的临床转归。结果微创手术加尿激酶血肿清除治疗组疗效明显优于对照组(P<0.05)。结论微创手术加尿激酶血肿清除治疗高血压脑出血,治疗组疗效好,可能是脑出血周围水肿脑组织水肿挤压减轻,坏死减少,全脑水肿减轻,并发症减少。脑内血肿对周边脑组织局部生化反应和毒性作用减轻,继发性神经原损伤减轻所致。